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Bioprocess Control

Bioreactor & fermentation operations · historian-linked

LIVE t+54.0 h
pH
7.169
DS 6.87.2
Dissolved O₂
17.2%
DS 2560%
Temperature
37.28°C
DS 36.237.4°C
Off-gas CO₂
6.33%
DS 0.56.5%
BR-2600 · CHO-K1 v4BR-2603 · P. pastoris X-33
Model cohort
10 runs
Specific growth µ
0.0012 h⁻¹
Projected final OD
87.6
Feed rate
42% confidence
81 mL/h89 mL/h(+8 mL/h)

Titer regresses on feed rate with slope 0.022 g/L per mL/h (R²=0.12) across 10 comparable runs.

Induction timing
37% confidence
29 h31 h(+2 h)

Top performer BR-2611 induced at 35% of run length (6.12 g/L). Timing slope 19.44 (R²=0.12).

DO setpoint
44% confidence
40 %45 %(+5 %)

Mean DO correlates with titer at slope 0.248 g/L per % (R²=0.24); stays within design space 25–60%.